FastQCFastQC Report
Mon 27 Feb 2023
SRR3128802.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128802.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1809259
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125790.6952570085322223No Hit
CTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGC29480.16293963440281353No Hit
CCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTG28120.15542274489169322No Hit
GCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTG25100.13873082847729376No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT51100.080.1273651
ACGGGTA3150.076.330984
CGTAGGG4200.074.085942
TAGAGGG31450.068.9572
AGGGATG27100.068.495575
AGGGCGA8600.068.304015
GAATAGG12600.068.136981
AGTAAGG12500.067.927331
AGAGGGA45700.067.881423
ATAGCGG3900.067.73381
TAGGGAT13200.067.503934
TAAGGGA18900.067.350853
TATAGGG12300.067.077072
AAGAGGG64100.066.783472
AGAGGGC24350.066.410433
ATAGGGC10050.066.144573
AGGGTAC8850.065.843525
CGAAGGG17750.065.605432
GAGGGAT25600.065.561854
GAGGGTA11400.065.342154
Produced by FastQC (version 0.11.5)