FastQCFastQC Report
Mon 27 Feb 2023
SRR3128801.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128801.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1874399
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121350.6474075156890289No Hit
CCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTG31990.17066803812848813No Hit
CTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGC31660.16890747380893822No Hit
GCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTG27650.14751394980471072No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT53550.081.0968551
CGTAGGG5350.072.950962
AGGGCAT16550.071.845155
TAGGGCA10600.071.82644
TAAGGGA16550.070.141313
GAATAGG12350.070.099141
ATAGGGC12250.069.441233
GTAGGGA14550.068.4774863
TAGAGGG32450.068.1067052
AGGGCAC15050.068.076145
AGGGATG26450.066.2762765
ACGGGTA2200.066.223784
TAGGGAT14200.066.193694
AAGGGCG8800.065.689714
ACGGGAT4150.065.683284
ATAGGGA16050.065.591553
CGAAGGG20450.065.532732
AGGGAAT20450.065.4977655
AGTAAGG13350.065.20071
AGAGGGA45750.065.128813
Produced by FastQC (version 0.11.5)