FastQCFastQC Report
Mon 27 Feb 2023
SRR3128800.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128800.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1990780
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125200.6288992254292287No Hit
CCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTG32270.16209726840735794No Hit
CTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGC32270.16209726840735794No Hit
GCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTG29290.14712826128452164No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT51450.080.121121
AAGAGGG72000.070.825672
GAATAGG13550.069.804761
ATAGGGC12750.069.669883
TAGCGGG10750.069.078372
AGGGATG29350.068.852725
ATAGGGA17950.068.6009753
AGTAGGG29600.067.799992
CGTAGGG5550.067.747062
TAGGGCA10900.067.6948554
TAAGGGA18550.067.142183
AGTAAGG14100.067.081881
AAGGGAT34700.065.824774
TAGAGGG33300.065.771112
CGAAGGG20350.065.591482
TATAGGG14300.065.076262
TAGGGAT14150.064.768034
TAGACGG2550.064.5886461
AGATAGG10950.064.462251
AGAGGGC28300.064.437513
Produced by FastQC (version 0.11.5)