FastQCFastQC Report
Mon 27 Feb 2023
SRR3128799.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128799.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2472822
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT384631.5554293839184543No Hit
CCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTG42370.17134270076859556No Hit
CTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTGC35410.1431967201844694No Hit
CGCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCT32460.13126703013803662No Hit
CGTTCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTT24770.1001689567627593No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT159350.086.08421
TAGGGCA13350.071.3627244
ACGGGTA2050.071.319644
AGGGATG36150.070.97595
GTAGGGC13300.070.567183
GAATAGG13450.070.51871
ATAGGGC12800.070.376023
GTAGGGA15350.068.5169143
ATAGGGA22550.067.7641143
TATAGGG18400.066.643362
AAGGGTA17650.066.535894
GAGGGAT31100.066.422474
AGTAGGG39700.066.408342
ATAGAGG17250.065.926261
TAGAGGG45600.065.8833852
AAGGGAT37600.065.727144
CGTAGGG7750.065.723912
GGGCGAT12950.064.954596
AGTAAGG15550.064.940241
AGACGGG16450.064.795352
Produced by FastQC (version 0.11.5)