FastQCFastQC Report
Mon 27 Feb 2023
SRR3128798.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128798.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2534192
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT380351.5008728620404452No Hit
CCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTG44140.1741778049966222No Hit
CTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTGC37450.14777885811335525No Hit
CGCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCT35150.13870298698756842No Hit
CGTTCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTT25550.10082109011471901No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT162850.086.940741
ATAGGGC12250.074.428393
AGGGATG35850.068.693415
ATAGGGA21150.067.99613
TAGGGCA13050.067.704934
GAATAGG14400.067.661421
TAGACGG3550.067.619991
AGTAAGG16900.066.8432851
AGTAGGG38150.066.196592
TAGAGGG44150.066.041482
TGTAGGG18050.065.6567152
AAGAGGG96150.065.34512
GATAGGG37550.065.125222
ACGGGTA2250.064.751854
TAGGGTA7700.064.0872344
TATAGGG18800.064.0380252
AGAGGGC37950.064.025323
GTAGGGC13000.063.9886363
AGGGCAT19800.063.8498765
GGTAAGG15200.063.790631
Produced by FastQC (version 0.11.5)