FastQCFastQC Report
Mon 27 Feb 2023
SRR3128797.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128797.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2665843
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT389581.4613763826301849No Hit
CCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTG47580.1784801280495513No Hit
CTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTGC39620.14862090528211902No Hit
CGCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCT36510.13695480191444132No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT159600.086.235021
ATAGGGC13050.073.830393
ATAGAGG18100.070.685861
TAGGGCA12800.070.4990164
ATAGCGG5750.069.533371
TACGGGT1850.068.5936363
GAATAGG15450.067.8921
ATAGGGA22050.067.5682143
CGTAGGG8350.067.54272
AGTAGGG40050.067.4759452
AGGGATG36150.067.4724355
GTAGGGC12950.067.1419143
GGTAAGG16400.066.8273541
AGATAGG15650.066.7238161
AAGGGAT40700.066.6305164
TAGAGGG50100.066.604612
TAAGGGT10650.066.196263
TAGGGCG5150.065.707824
TATAGGG18900.065.399982
AGAGGGC40500.064.986753
Produced by FastQC (version 0.11.5)