FastQCFastQC Report
Mon 27 Feb 2023
SRR3128796.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128796.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences179732
Sequences flagged as poor quality0
Sequence length100
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGC20171.1222264260120625TruSeq Adapter, Index 14 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTG18501.0293103064562794No Hit
GCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTG13640.7589077070304676No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7290.4056038991387177No Hit
GCAGAGAATATCAACTGTCTCTTATACACATCTGACGCCTAATGACTCGT5430.30211648454365386No Hit
CGCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCT5350.2976654129481673No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA4920.2737409031224267No Hit
AGCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCT4290.23868871430796965No Hit
ACTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTG3950.21977166002715154No Hit
GCCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCT3770.20975674893730667No Hit
GGCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCT3150.1752609440722854No Hit
GCAGCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTT3090.17192264037567045No Hit
GCAGAGACTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGT3090.17192264037567045No Hit
AGAGCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTT3070.1708098724767988No Hit
GCAGAGAATATCCTGTCTCTTATACACATCTGACGCCTAATGACTCGTAT2870.15968219348808224No Hit
AAAGCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTT2860.1591258095386464No Hit
AAATCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTT2780.1546747379431598No Hit
GCAGAGAATATCAAAGCGGAAAAAATCCAAAAAAAAAAAAAAAAAAAAAA2770.15411835399372398No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG2700.1502236663476732No Hit
GAAGCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTT2670.14855451449936574No Hit
GGAGCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTT2520.14020875525782833No Hit
ACCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCT2510.1396523713083925No Hit
TCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTG2350.13075022811741926No Hit
GGAACTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTT2340.13019384416798346No Hit
CGTTCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTT2200.12240446887588187No Hit
GAAAAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA2100.1168406293815236No Hit
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT2080.11572786148265195No Hit
GGACCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTT2070.11517147753321612No Hit
AAGACTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTT1980.11016402198829368No Hit
AGAAAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA1930.10738210224111454No Hit
ATAGCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTT1880.10460018249393542No Hit
GAATCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTT1850.10293103064562793No Hit
AAAACTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTT1830.10181826274675627No Hit
AGAACTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTT1800.1001491108984488No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTAAGG254.8186666E-794.2779851
GCATAGG550.094.2779851
GTACCGG156.812205E-494.277981
CATGCGG156.812205E-494.277981
GACACGG156.812205E-494.277981
TCGTGGG156.812205E-494.277981
AGTACGG156.812205E-494.277981
AAGGGTC254.83471E-794.225394
CCACGGG254.83471E-794.225392
ATGTTCA254.83471E-794.225394
ACGGGTA156.827337E-494.225384
ACGGGAT201.8119767E-594.225384
CAAGGGT409.094947E-1294.225383
TAGGGAG550.094.225384
CACCGGG156.827337E-494.225382
TACCGGG156.827337E-494.225382
GGTCCAT201.8346856E-593.989437
TCTAGTT201.8346856E-593.989439
GCATGCT409.094947E-1293.989438
ATACCGG201.8346856E-593.989439
Produced by FastQC (version 0.11.5)