FastQCFastQC Report
Mon 27 Feb 2023
SRR3128795.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128795.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences186476
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGC21401.1476007636371437TruSeq Adapter, Index 14 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTG19411.040884617859671No Hit
GCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTG14910.7995667002724212No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7410.3973701709603381No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCCTAATGAC6300.3378450846221498No Hit
CGCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCT5990.32122096141058365No Hit
GCAGAGAATATCAACTGTCTCTTATACACATCTGACGCCTAATGACTCGT5120.27456616401038203No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA4570.24507175186082927No Hit
ACTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTG4510.24185417962633263No Hit
AGCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCT4250.2279113666101804No Hit
GGCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCT3900.2091421952422832No Hit
GCCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCT3740.20056200261695875No Hit
GCAGCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTT3250.17428516270190267No Hit
GCAGAGACTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGT3140.1683862802719921No Hit
AAAGCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTT3100.166241232115661No Hit
AGAGCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTT3030.16248739784208155No Hit
GCAGAGAATATCCTGTCTCTTATACACATCTGACGCCTAATGACTCGTAT2940.15766103949033655No Hit
GGAGCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTT2930.15712477745125378No Hit
GCAGAGAATATCAAAGCGGAAAAAATCCAAAAAAAAAAAAAAAAAAAAAA2930.15712477745125378No Hit
GAAGCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTT2790.1496171089040949No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG2720.14586327463051546No Hit
ACCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCT2660.1426457023960188No Hit
AAATCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTT2630.14103691627877046No Hit
TCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTG2600.13942813016152214No Hit
GGAACTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTT2490.13352924773161157No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA2470.13245672365344602No Hit
AAGACTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTT2370.12709410326261825No Hit
ATAGCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTT2270.12173148287179048No Hit
AGAACTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTT2210.1185139106372938No Hit
CGTTCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTT2170.11636886248096269No Hit
GGACCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTT2140.11476007636371437No Hit
GAAACTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTT2090.11207876616830048No Hit
GAATCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTT2020.10832493189472103No Hit
AATGCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTT1970.10564362169930715No Hit
AAAACTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTT1930.10349857354297605No Hit
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT1870.10028100130847938No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGAGG201.8218416E-594.124331
GTACCGG156.8569375E-494.124331
ATAGCGG156.8569375E-494.124331
TTGTAGG156.8569375E-494.124331
CTAACGG156.8569375E-494.124331
GTATAGG750.094.124331
GATTAGG201.8218416E-594.124331
AGTACGG254.8664515E-794.124331
AGTAAGG900.094.124321
TACCGGG156.878928E-494.048572
TCGCGGG201.8291435E-594.048562
CGGGTAC254.9055234E-793.998125
GGTACGC201.8340244E-593.998127
GGCAATG201.8340244E-593.998126
GGGTACG550.093.998126
AGGGTCC409.094947E-1293.998125
ACGGGTC156.893617E-493.998124
AGCGGGT254.9055234E-793.998123
CGAGGGA201.8340244E-593.998123
TAGGGTC500.093.998124
Produced by FastQC (version 0.11.5)