FastQCFastQC Report
Mon 27 Feb 2023
SRR3128794.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128794.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences187786
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGC22191.181664234820487TruSeq Adapter, Index 14 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTG20411.0868754859254683No Hit
GCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTG15390.819549913199067No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7340.3908704589266505No Hit
CGCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCT6020.3205776788471984No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA5070.2699881780324412No Hit
GCAGAGAATATCAACTGTCTCTTATACACATCTGACGCCTAATGACTCGT4760.25348002513499407No Hit
AGCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCT4520.2406995196660028No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCCTAATGAC4220.22472388782976369No Hit
GCCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCT4200.22365884570734773No Hit
ACTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTG3880.20661817174869265No Hit
GGCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCT3380.17999211868829412No Hit
GCAGAGACTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGT3290.17519942913742237No Hit
AGAGCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTT3260.17360186595379848No Hit
GCAGCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTT3240.17253682383138255No Hit
GAAGCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTT3130.16667909215809484No Hit
TCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTG2940.1565611919951434No Hit
AAAGCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTT2890.15389858668910356No Hit
GCAGAGAATATCCTGTCTCTTATACACATCTGACGCCTAATGACTCGTAT2870.1528335445666876No Hit
ACCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCT2860.15230102350547964No Hit
AAATCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTT2710.14431320758736008No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG2700.1437806865261521No Hit
GCAGAGAATATCAAAGCGGAAAAAATCCAAAAAAAAAAAAAAAAAAAAAA2570.13685791273044848No Hit
GGACCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTT2540.1352603495468246No Hit
GGAACTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTT2450.13046765999595283No Hit
AAGACTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTT2340.12460992832266517No Hit
AAAACTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTT2340.12460992832266517No Hit
ATAGCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTT2300.12247984407783327No Hit
CGTTCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTT2290.12194732301662531No Hit
AGAACTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTT2240.11928471771058546No Hit
GAATCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTT2070.11023185967004995No Hit
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT2020.10756925436401009No Hit
GAAACTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTT2020.10756925436401009No Hit
AATGCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTT2000.10650421224159415No Hit
GAAAAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA1970.10490664905797024No Hit
GGAGCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTT1960.10437412799676228No Hit
AGAAAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA1910.10171152269072242No Hit
AGGGCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTT1900.10117900162951446No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGAGG353.5470293E-1094.057841
GCGTAGG156.876306E-494.057841
GGCTAGG156.876306E-494.057841
AGCACGG156.876306E-494.057841
GATAACT254.910453E-793.98278
AATGGGC500.093.98273
CAAGGGT500.093.98273
GGGATCC500.093.98276
GTTGAGC156.8981934E-493.982699
ATGGGCC301.3214958E-893.982694
CCGGGAT156.8981934E-493.982694
GTCCTGA156.8981934E-493.982697
GGTTTGC156.8981934E-493.982697
TAACGGG156.8981934E-493.982692
GTAATTG156.8981934E-493.982698
GAGCGAT201.8355538E-593.982698
CGGGATA156.8981934E-493.982695
GACGGTC156.8981934E-493.982693
GTATCAC156.8981934E-493.982698
GGCATGC156.8981934E-493.982697
Produced by FastQC (version 0.11.5)