FastQCFastQC Report
Mon 27 Feb 2023
SRR3128793.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128793.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2367617
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT171720.7252862266152No Hit
CTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGC33460.14132353332485786TruSeq Adapter, Index 16 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTG33200.14022538273715723No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT74800.081.484111
ATAGGGC13700.076.759233
CGTAGGG6450.076.036112
ATAGCGG4850.072.951051
AGGGATG35200.071.2900545
AGTAGGG40900.071.139072
ATAGGGA20100.069.445163
AGTAAGG15150.068.816351
CGAAGGG23950.068.717322
GAATAGG15400.068.6181951
TAGGGAT17000.068.2389764
AAGAGGG91800.067.242412
GTAGGGA20450.067.103623
TAGAGGG43400.066.823882
AGAGGGA58300.066.650743
AGGGCAT20750.066.355945
TAGGGCA10550.066.1539154
TAAGGGA21500.065.800483
GGTAAGG14850.065.441431
TAGGGTC5850.065.294334
Produced by FastQC (version 0.11.5)