FastQCFastQC Report
Mon 27 Feb 2023
SRR3128792.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128792.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2440987
Sequences flagged as poor quality0
Sequence length100
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT168650.6909090462177799No Hit
CTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGC35340.14477750188755614TruSeq Adapter, Index 16 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTG34760.14240141385431385No Hit
GCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTG26920.11028325837048703No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT76250.079.739561
ATAGCGG4800.071.5703051
GAATAGG15400.071.506631
AGGGATG36650.071.298815
AGTAGGG43400.069.471352
ATAGGGC13650.069.2061843
ATAGGGA22750.068.793013
ATAAGGG32900.068.196172
GTAGGGC11800.067.311023
GGTAAGG15200.067.1842351
GTAGGGA21700.066.923743
TAGAGGG44700.066.8195652
CGTAGGG7150.066.443542
AAGAGGG92450.066.3958742
AGGGCAT19650.066.012785
TAGGGAT17600.065.957744
TAGGGCA12550.065.535384
AAGGGAT38750.065.251744
AAGGGGC77200.065.0791554
ACTAGGG12600.064.9555362
Produced by FastQC (version 0.11.5)