FastQCFastQC Report
Mon 27 Feb 2023
SRR3128791.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128791.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2569615
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT176290.6860560823313998No Hit
CTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGC37520.14601409160516265TruSeq Adapter, Index 16 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTG35710.13897023484062787No Hit
GCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTG26670.10378986735366971No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT80950.079.103691
ATAGCGG4700.077.0811841
AGGGATG36850.071.5426565
ATAGGGC14050.071.2431263
AGTAGGG44350.070.358112
TAGAGGG47300.069.248652
ATAGGGA20400.069.108393
GAATAGG14200.068.917651
ATAAGGG31850.068.609342
TATAGGG20000.067.2010042
TAGGGCA11200.067.1338654
TGTAGGG18800.066.2411352
AAGGGGC80400.066.165274
AGATAGG14300.066.132581
AAGAGGG98600.066.058092
AGTAAGG15100.065.7447361
TAAGGGA19250.065.669123
TAGCGGG14900.065.286552
ATGAGGG43800.064.804112
AAGGGAT42100.064.518674
Produced by FastQC (version 0.11.5)