FastQCFastQC Report
Mon 27 Feb 2023
SRR3128790.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128790.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2333467
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147230.6309495698889249No Hit
CTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGC38840.16644760778703963No Hit
CCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTG30120.12907831994195762No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC30030.128692627750896No Hit
GCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTG26660.11425059793003287No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT66500.078.978451
AGGGATG43400.077.959455
TAGGGCA13350.074.198584
GGTAAGG13850.071.8898851
GAATAGG15900.071.524521
AAGGGAT46250.071.0851064
CGTAGGG8900.071.018642
ATAGGGA21950.070.485033
TAAGGGA21350.070.2565463
ATAGGGC12600.070.004933
ATGCGGG12550.069.907992
TAGACGG2500.069.8388061
TATAGGG17150.069.584732
GAGGGAT35600.069.5611654
AGTAGGG38450.069.066842
AGTAAGG13750.068.637641
TAGAGGG41200.068.578372
GTAGGGA22750.068.421113
ATAGAGG15900.068.259921
AGGGTAC11650.066.958885
Produced by FastQC (version 0.11.5)