FastQCFastQC Report
Mon 27 Feb 2023
SRR3128789.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128789.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2387439
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145560.6096909701148385No Hit
CTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGC38950.16314552958211706No Hit
CCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTG33350.1396894329027883No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC30750.12879910230167138No Hit
GCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTG27300.11434847131172776No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT68250.079.6927951
AGGGATG49000.077.9770665
ATAGGGA23550.074.0381243
AAGGGAT50950.073.240074
CGTAGGG7700.072.670162
TAGGGCA12300.071.4510964
GAGGGAT34250.069.157994
AGTAGGG37500.068.463842
GGATGGC38550.067.905237
AGAGGGC35200.067.6920553
TAGACGG2850.067.686621
GAATAGG14400.067.635031
GGTAAGG12150.067.3809051
GTAGGGC13350.067.239513
ATAGAGG18400.066.995761
AAGAGGG88650.066.939192
ATGCGGG11450.066.5287552
CGAAGGG24600.066.1364442
TAACGGG6850.065.899262
AGTAAGG15150.065.8395841
Produced by FastQC (version 0.11.5)