FastQCFastQC Report
Mon 27 Feb 2023
SRR3128788.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128788.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2487612
Sequences flagged as poor quality0
Sequence length100
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149750.6019829458934914No Hit
CTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTGC41110.16525889085596948No Hit
CCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTG33540.13482810020212155No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC31900.12823543221370534No Hit
GCTGTCTCTTATACACATCTGACGCACTTTGCGTCGTATGCCGTCTTCTG29560.11882882057169686No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT66800.079.674061
CGTAGGG7000.078.549252
AGGGATG45850.075.845385
GAATAGG14900.074.218531
ATAGGGA22000.073.6971363
ATAGCGG6100.072.515161
AAGGGAT50350.071.869644
TAGGGCA12100.071.852284
AGTAAGG14750.071.782941
GAGGGAT36500.068.754734
TAGAGGG44550.068.462262
TAGACGG3200.067.6454541
GGTAAGG13250.067.478971
ATAGGGC14150.067.4207843
AATAGGG40450.067.385072
AGGGAAT27300.067.133415
AGTAGGG38750.066.945532
AGACGGG17000.066.899162
AGAGGGC36050.066.614663
AAGAGGG94350.066.545512
Produced by FastQC (version 0.11.5)