FastQCFastQC Report
Mon 27 Feb 2023
SRR3128787.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128787.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2296760
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT207930.9053187969139136No Hit
CTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTGC34240.14907957296365315No Hit
CCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTG32020.13941378289416395No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT80900.080.297021
CGTAGGG6600.072.165592
GAATAGG13150.071.390651
TAGTAGG8800.071.298931
TATAGGG17500.070.601832
ATAGGGA18200.070.4774553
AAGGGGC87300.069.197054
AGTAGGG38700.068.116692
TAGGGCA9700.068.0626754
ATAGGGC12000.066.806763
ATAGAGG15450.066.259061
AGGGGCT107050.066.197645
TAGAGGG40550.066.1719742
AGTAAGG14200.066.111771
AGATAGG11850.066.085141
ATGTAGG7500.066.045331
AGGGCAT17250.065.108345
ATAAGGG29650.064.7325062
GTAGGGA15700.064.5790253
GGTAAGG12550.064.2785951
Produced by FastQC (version 0.11.5)