FastQCFastQC Report
Mon 27 Feb 2023
SRR3128786.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128786.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2389143
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT203900.8534441010856195No Hit
CTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTGC38100.15947140878549337No Hit
CCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTG33630.14076177106184098No Hit
GCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTG24090.10083113484626077No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT84750.081.113621
GAATAGG13350.075.0726241
ATAGCGG6050.069.9955441
AGTAGGG40550.069.573442
ATAGGGC11850.068.217093
TAGTAGG8250.067.869751
TAGGGCA10050.067.808634
AGGGCAT18450.067.759215
AGTAAGG16800.067.498021
AGATAGG12350.067.435651
CGTAGGG6800.067.0727162
ATAGGGA18100.066.9921953
GGTAAGG14050.066.978741
AAGGGGC94300.066.286194
TAGAGGG42850.066.277962
ATGTAGG7700.065.99581
AGGGTAC10000.065.797765
TAGGGTA5600.065.462054
AAGGGTA14400.065.275554
AGGGGCT113000.065.2570655
Produced by FastQC (version 0.11.5)