FastQCFastQC Report
Mon 27 Feb 2023
SRR3128785.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128785.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2518742
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT209330.8310894883239331No Hit
CTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTGC39630.15734045011358846No Hit
CCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTG35580.14126099457586366No Hit
GCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTG26530.10533035936193544No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT81750.079.080231
GAATAGG15100.077.275831
ATGTAGG7400.075.027291
ATAGGGA18650.073.076743
TAGTAGG9250.072.229661
ATAGCGG6450.072.217831
ATAGGGT9150.068.82463
AGATAGG14350.068.527261
AAGGGGC102500.068.49944
GTATAGG8450.068.488461
AGTAGGG45350.068.18812
AGTAAGG16350.067.3390661
ATAGGGC12800.067.189453
AAGGGAC23250.067.108124
GATAGGG37600.066.4941942
GGTAAGG16050.066.252511
TAGGGTA6600.066.22154
TATAGGG19150.065.769722
GTAGGGT7500.065.167653
AGGGGCT118350.065.161595
Produced by FastQC (version 0.11.5)