FastQCFastQC Report
Mon 27 Feb 2023
SRR3128784.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128784.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1901074
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160900.8463636870526872No Hit
CTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTGC28800.15149331377947414TruSeq Adapter, Index 16 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTG26770.1408151392318237TruSeq Adapter, Index 13 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTG23200.1220362805445764TruSeq Adapter, Index 13 (95% over 21bp)
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC20250.10651873625119274No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT60650.080.548331
TAAGGGA17550.072.7863
AGGGATG31900.072.0374455
ATAGGGA18750.070.138853
GGTAAGG10900.070.083561
TAGGGTA5950.069.714234
ATGTAGG5800.069.106441
ATAGAGG13900.068.188141
GTAGGGC7400.068.156583
AGTAGGG29600.067.997332
GAGGGAT24550.067.968474
AGATAGG10700.067.867921
TAGGGCA8000.067.758474
TAGAGGG37000.067.51962
AGTACGG2800.067.3642651
AGTAAGG13700.067.118411
CGTAGGG5000.066.9335862
AGAGGGA44400.066.776453
GAATAGG11300.066.350821
ATAGGGC10800.066.340013
Produced by FastQC (version 0.11.5)