FastQCFastQC Report
Mon 27 Feb 2023
SRR3128783.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128783.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1955571
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT162270.829783219325711No Hit
CTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTGC32550.1664475490790158TruSeq Adapter, Index 16 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTG30040.15361242317461243TruSeq Adapter, Index 13 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTG24260.12405583842263973TruSeq Adapter, Index 13 (95% over 21bp)
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC20630.10549348502304443No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT57200.081.605181
AGGGATG31150.072.571645
TAGGGCA7700.071.4126364
GAGGGAT24700.070.78254
GTAGGGC6750.070.323013
CGAAGGG18150.070.205722
GACCGAT1550.069.739058
TAATAGG6900.069.558981
CGTAGGG5650.069.073182
TAGGGTA7100.068.8422244
AGTAAGG11650.068.663371
ATAGGGC11000.068.360813
GAATAGG11750.068.0789951
AGTAGGG27300.067.859932
ATAGAGG13050.067.787471
ACGGGTA1600.067.559714
AGGGCAT14100.066.663915
TAGAGGG34650.066.49252
AGAAGGG65900.065.927462
ATGTAGG6650.065.805691
Produced by FastQC (version 0.11.5)