FastQCFastQC Report
Mon 27 Feb 2023
SRR3128782.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128782.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2041034
Sequences flagged as poor quality0
Sequence length100
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT162940.7983208510980219No Hit
CTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTGC33050.1619277287884474TruSeq Adapter, Index 16 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTG30820.15100189413797124TruSeq Adapter, Index 13 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTG27380.13414769180719185TruSeq Adapter, Index 13 (95% over 21bp)
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC21700.10631866005171887No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT63450.081.7782061
TAGGGCA7800.074.108454
AGGGATG33600.072.311685
CGTAGGG4100.072.209222
ATAGGGC10400.071.397173
GTAGGGC8000.071.080853
AGTAAGG13250.070.2980041
TAGGGTA5750.070.289084
GTAGGGA14450.070.249483
AGTACGG3350.070.213221
GAGGGAT25850.070.1752554
ATAGAGG11700.069.55911
GAATAGG12500.068.494391
AGGGTAC10200.068.18975
GGTAAGG12600.067.950791
TAAGAGG15200.067.469361
AGAGGGA48900.067.369973
TAAGGGA16250.067.095263
TAGGGCG4000.066.9687354
AGTAGGG32600.066.886162
Produced by FastQC (version 0.11.5)