FastQCFastQC Report
Mon 27 Feb 2023
SRR3128781.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128781.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2341741
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT266511.138084869334397No Hit
CCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTG27150.11593938014494345No Hit
CTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGC25520.10897874700916968Illumina Single End Adapter 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT101700.084.056191
AGGGATG38550.072.774985
TAAGGGA22050.070.999813
AAGGGAT38350.068.73444
AGATAGG15350.068.5376051
ATAGCGG4550.068.432911
AGTAAGG14950.067.846851
ATAGGGA23050.067.510413
AGTAGGG38550.067.399222
TAGAGGG41950.067.332162
GTAGGGT7500.067.274463
ATAGGGC13100.066.952893
ATAGAGG17700.066.634511
AGGGAAT23350.066.615195
GTAGGGC11400.066.596093
TAGGGCA11050.066.145014
GGTAAGG14300.065.652231
CGAAGGG23850.065.6413352
TAACGGG7300.065.2418442
TAGGGTA5650.065.098934
Produced by FastQC (version 0.11.5)