FastQCFastQC Report
Mon 27 Feb 2023
SRR3128780.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128780.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2381491
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT264621.111152635050899No Hit
CCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTG27690.11627169701670088No Hit
CTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGC26720.1121986184285391Illumina Single End Adapter 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT106900.084.55351
AGGGATG39550.071.535045
TAGAGGG40650.071.1712
ATAGGGC12450.070.967153
TAAGGGA21200.070.938663
AGTAAGG15400.069.392611
AAGGGAT39850.068.637834
GAGGGTA13150.068.26164
AGATAGG16750.066.891391
AAGAGGG89250.066.7289052
ATAGCGG5300.066.6182861
ATAGGGA23000.066.204283
GATAGGG36750.065.6672062
AGGGAAT22700.065.422945
GGATAGG13150.065.155891
TAAGAGG17750.064.9794161
AGAGGGA54250.064.5394743
GGTAAGG13350.064.532421
ATGCGGG10950.064.44152
GAATAGG15700.064.168541
Produced by FastQC (version 0.11.5)