FastQCFastQC Report
Mon 27 Feb 2023
SRR3128779.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128779.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2477451
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT269031.0859145145554847No Hit
CCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTG30480.12302967848809118No Hit
CTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGC30190.12185912052347353Illumina Single End Adapter 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT106050.083.53771
AGGGATG40050.070.753675
ATAGGGC12650.070.213883
AGATAGG14800.069.30071
TAGAGGG44550.068.9892962
AGGGTAC9750.067.959265
ATAGGGA22400.067.555313
GTACGGG5350.066.7592542
AAGAGGG96600.065.968152
GTAGGGT7850.065.8528753
AAGGGTA15500.065.793014
GAGGGAT31400.065.553544
ATAGAGG20100.065.305681
TAAGGGA21500.064.918683
TAAGAGG19900.064.7799151
AGAAGGG94050.064.7086942
ACTAGGG12650.064.641352
CGAAGGG27400.064.48952
TATAGGG18100.064.3909452
ATAAGGG36000.064.357052
Produced by FastQC (version 0.11.5)