FastQCFastQC Report
Mon 27 Feb 2023
SRR3128778.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128778.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1988813
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT251251.2633163600599957No Hit
CCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTG23870.12002133936171977No Hit
CTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGC22860.11494293329739899No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT98600.083.872691
ATAGGGC12550.073.652153
GAATAGG13300.070.9404451
AGGGCAT17100.069.802095
ACGGGTA3250.069.651574
GAGGGTA11450.069.6072164
AGGGATG30750.069.0755165
ATAGGGA16350.068.64873
AGGGTAC8850.068.49795
ATAGCGG4700.068.253761
TAGAGGG39200.068.0931552
TAAGGGA16850.067.731163
ATAAGGG29050.067.533652
AGATAGG11350.067.3340451
AAGAGGG73600.067.2157062
AGTACGG2600.067.134221
TAGCGGG11350.066.480942
GGATAGG13650.066.35661
AAGGGCA30800.066.29954
GATAGGG31000.066.176022
Produced by FastQC (version 0.11.5)