FastQCFastQC Report
Mon 27 Feb 2023
SRR3128777.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128777.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2009995
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT240931.1986596981584532No Hit
CCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTG25990.129303804238319No Hit
CTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGC23510.11696546508822162No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT95650.084.5915761
ACGGGTA3200.074.903814
CGTAGGG6500.073.7808152
ATAGGGA17850.071.880013
AGGGATG31050.071.2923665
ATAGAGG14900.070.404791
TAGGGCA9650.067.697274
GAATAGG14950.067.337381
TAGAGGG39350.067.269822
ATAAGGG27600.066.948392
ATAGGGC12700.066.612013
AGGGTAC11600.066.446115
GGTAAGG12250.066.4344941
ATAGCGG4200.066.082481
AATAGGG33200.065.852382
AGTAGGG32300.065.649382
TATAGGG14800.064.807472
TAGGGTC5150.064.794014
AAGGGCG8200.064.766184
AAGGGCA29100.064.764214
Produced by FastQC (version 0.11.5)