FastQCFastQC Report
Mon 27 Feb 2023
SRR3128776.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128776.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2131087
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT249001.168417807438176No Hit
CCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTG27400.12857288322813662No Hit
CTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGC24950.11707640279350397No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT97350.084.438151
TAGGGTA6600.079.0425954
ATAGGGA17400.073.19853
TAGGGCA9400.071.4974064
ATAGAGG15250.070.347191
CGTAGGG6900.070.156882
GAATAGG17050.069.543721
TATAGGG16900.069.246012
AGATAGG14000.069.234241
ATAGGGC11750.069.1974953
GATAGGG34100.069.050292
GGTAAGG13500.069.010181
ACGGGTA3550.068.8425754
AGGGTAC11200.068.815715
ATAAGGG27300.068.689832
TAGAGGG40650.068.676482
AATAGGG35400.066.9128342
AGTAGGG39450.066.834082
AGGGATG32450.066.764865
GTAGGGC10150.066.2143553
Produced by FastQC (version 0.11.5)