FastQCFastQC Report
Mon 27 Feb 2023
SRR3128775.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128775.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences162111
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGC11030.6803979989019869TruSeq Adapter, Index 22 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTG11030.6803979989019869No Hit
GCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTG8820.5440716546070286No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6700.4132970618896929No Hit
CGCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCT3710.2288555372553374No Hit
AGAGCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTT3270.20171364065362624No Hit
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT3250.20047991808082116No Hit
AAAGCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTT3140.19369444393039337No Hit
AGCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCT3010.18567524720716053No Hit
ACTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTG3000.185058385920758TruSeq Adapter, Index 22 (95% over 21bp)
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA2800.17272116019270745No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG2670.16470196346947463No Hit
GGCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCT2390.14742984745020388No Hit
AGATCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTT2280.14064437329977608No Hit
AGGGCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTT2210.13632634429495838No Hit
ACCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCT2180.1344757604357508No Hit
GAAGCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTT2030.12522284113971294No Hit
AAGCCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTT1950.1202879508484927No Hit
GCCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCT1860.11473619927086998No Hit
GAATCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTT1800.11103503155245481No Hit
AGGCCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTT1790.11041817026605227No Hit
ATAGCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTT1750.10795072512044215No Hit
AATGCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTT1740.10733386383403964No Hit
GGAACTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTT1720.10610014126123457No Hit
TCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTG1690.104249557402027No Hit
GAAACTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTT1680.10363269611562448No Hit
GGAGCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTT1680.10363269611562448No Hit
GTAGAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA1670.10301583482922194No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATAGG700.094.33531
TGACCGG156.7945453E-494.33531
TGGATGG254.7990943E-794.33531
GCATCGG156.7945453E-494.33531
TCGTAGG201.8009767E-594.335291
AGTAAGG450.094.335291
TACGGGT156.802914E-494.30613
CATAGGC156.802914E-494.30612
GCGGGTA156.802914E-494.30614
TAGGGTA254.807953E-794.30614
TTACGGG156.802914E-494.30612
TAAGGGT301.2892997E-894.30613
CCTTGTA156.802914E-494.30614
ACGGGGA201.8037483E-594.306094
CGTAGGG409.094947E-1294.306092
CGGGTAG201.8344612E-593.986125
GGCACCG301.3200406E-893.986127
GGATCGG156.8954774E-493.986128
AGGGTCG201.8344612E-593.986125
GGATACG409.094947E-1293.986128
Produced by FastQC (version 0.11.5)