FastQCFastQC Report
Mon 27 Feb 2023
SRR3128774.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128774.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences169177
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTG12260.7246847975788671No Hit
CTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGC12000.7093162782174882TruSeq Adapter, Index 22 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTG10080.5958256737026901No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6980.41258563516317226No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCAAAGGTCC5150.3044149027350054No Hit
AGCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCT4320.25535386015829575No Hit
CGCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCT3890.2299366935221691No Hit
AAAGCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTT3820.22579901523256707No Hit
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT3470.20511062378455702No Hit
AGAGCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTT3340.19742636410386757No Hit
ACTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTG3050.1802845540469449TruSeq Adapter, Index 22 (95% over 21bp)
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA2820.16668932538110973No Hit
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG2580.15250299981675997No Hit
GAAGCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTT2390.14127215874498306No Hit
AGGGCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTT2370.14008996494795392No Hit
AGATCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTT2160.12767693007914788No Hit
ACCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCT2150.1270858331806333No Hit
GGCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCT2060.12176596109400215No Hit
GGAGCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTT1960.1158549921088564No Hit
GGAACTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTT1940.11467279831182725No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA1920.1134906045147981No Hit
AGGCCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTT1900.11230841071776895No Hit
AAGCCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTT1860.10994402312371067No Hit
GTAGAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA1840.10876182932668152No Hit
GCCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCT1820.10757963552965237No Hit
ATAGCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTT1810.1069885386311378No Hit
GAAACTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTT1790.10580634483410867No Hit
AATGCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTT1780.10521524793559407No Hit
AAGACTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTT1770.10462415103707952No Hit
AGGACTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTT1730.10225976344302122No Hit
GCAGAGAATATCAACTGTCTCTTATACACATCTGACGCAAAGGTCCTCGT1710.10107756964599207No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTAAGG850.094.163761
ATAGCGG254.8523543E-794.163761
AATACGG201.8175646E-594.163761
CGTTCTG156.84442E-494.163751
TGACCGG156.84442E-494.163751
GCACGGG301.3058525E-894.135882
CGGGTAG450.093.996755
CGGGAAT450.093.996755
TAAGGGT450.093.996753
CTAGGGC201.833663E-593.996753
GGTAGTA201.833663E-593.996757
AGGGTCG254.903959E-793.996745
TACGGGA156.8929023E-493.996743
GTAGCTA156.8929023E-493.996748
GCATTAG156.8929023E-493.996749
TAGGGCA301.319313E-893.996744
GCTAGTA156.8929023E-493.996747
GCGCTTA156.8929023E-493.996749
TGTCGGG156.8929023E-493.996743
GTAAAGG1250.090.39721
Produced by FastQC (version 0.11.5)