FastQCFastQC Report
Mon 27 Feb 2023
SRR3128773.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128773.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences174961
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTG13250.7573116294488486No Hit
CTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGC12590.7195889369630946TruSeq Adapter, Index 22 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTG10640.6081355273460942No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7020.40123227462120126No Hit
CGCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCT3940.2251930430210161No Hit
AGAGCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTT3840.21947748355347763No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCAAAGGTCC3820.2183343716599699No Hit
AAAGCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTT3790.21661970381970838No Hit
AGCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCT3780.21604814787295454No Hit
GGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGGTAT3540.20233080515086216No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA3160.18061167917421597No Hit
ACTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTG3110.17775389944044673TruSeq Adapter, Index 22 (95% over 21bp)
GGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGG2850.1628934448248467No Hit
AGGGCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTT2560.14631832236898507No Hit
GAAGCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTT2430.13888809506118507No Hit
GGCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCT2380.13603031532741583No Hit
ACCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCT2350.13431564748715427No Hit
AGATCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTT2200.12574230828584657No Hit
GCCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCT2140.12231297260532348No Hit
GGAACTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTT2100.12002674881830809No Hit
AAGCCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTT2080.11888363692480039No Hit
AGGACTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTT2030.11602585719103113No Hit
AATGCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTT2020.1154543012442773No Hit
GAAACTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTT2000.1143111893507696No Hit
GGAGCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTT1910.10916718582998496No Hit
ATAGCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTT1890.10802407393647727No Hit
AGAAAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA1870.10688096204296957No Hit
GTAGAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA1820.10402318230920034No Hit
AGGCCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTT1820.10402318230920034No Hit
AAATCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTT1810.10345162636244648No Hit
TCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTG1810.10345162636244648No Hit
AGAACTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTT1780.10173695852218495No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTAGG201.8268318E-594.069231
TAGTAGG201.8268318E-594.069231
TAGACGG301.31258275E-894.069221
ATAGCGG156.8722066E-494.069221
TAGCAGG353.5470293E-1094.069221
GATTAGG156.8722066E-494.069221
ATGGGTA156.887852E-494.015444
TCAGGGA156.887852E-494.015443
TAAGGGT201.8320292E-594.015443
GAGGGTA254.898866E-794.015444
CGTAGGG700.094.0154342
CGGGTAG156.895685E-493.988575
GATACCA156.895685E-493.988578
GGTCGGA156.895685E-493.988577
GAGTCAT156.895685E-493.988578
AGGGTCA156.895685E-493.988575
GCGATTG156.895685E-493.988578
GGGTTAC156.895685E-493.988577
GGCGATT156.895685E-493.988577
GTAACGA156.895685E-493.988578
Produced by FastQC (version 0.11.5)