FastQCFastQC Report
Mon 27 Feb 2023
SRR3128772.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128772.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1831686
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118980.6495654822933625No Hit
CTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGC33330.1819635024780448Illumina PCR Primer Index 2 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTG33080.180598639723184Illumina PCR Primer Index 2 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTG29100.15887002466579972Illumina PCR Primer Index 2 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT45050.078.534341
AGGGATG47350.078.006875
CGTAGGG4250.073.226742
TAAGGGA19800.073.111873
GAGGGAT29350.072.4574664
AAGGGAT40950.072.0833744
TAGAGGG36050.071.5478742
GTAGGGC9000.071.25433
GAATAGG12150.070.6623841
TAGGGCA10550.070.618644
TAGGGCG3350.070.378494
AGGGCAT18700.070.363385
GAGGGTA12550.070.260724
GGATGGC34300.069.87257
AAGGGCA32300.069.4894944
AAGGGTA16700.069.459774
AAGAGGG67600.069.195792
GGTAAGG12450.068.580771
ATAGCGG3650.068.497731
AGTAGGG30400.068.403722
Produced by FastQC (version 0.11.5)