FastQCFastQC Report
Mon 27 Feb 2023
SRR3128771.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128771.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1862797
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115280.6188543357112987No Hit
CCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTG35200.18896315594238128Illumina PCR Primer Index 2 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGC34870.18719162635542144Illumina PCR Primer Index 2 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTG30520.16383964543640558Illumina PCR Primer Index 2 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTACGG3050.081.767711
CGTTTTT43400.079.03941
AGGGATG45950.076.1973655
GTACGGG5550.075.415012
CGTAGGG6300.074.6483462
AGTAAGG12350.073.535351
AGGGTAT13550.072.8365255
ATAGCGG3250.072.39231
TAGGGTA7350.072.253734
GAGGGAT30400.071.732114
TAGAGGG34300.071.1596762
TAAGGGA19200.070.98493
GGTAAGG11150.070.476991
ATAGGGC11350.070.391833
ATAAGGG28550.070.3367842
GGATGGC34700.069.479577
AGAGGGC23100.069.376373
TAGGGCA10600.069.165254
GAATAGG13550.069.1066061
AAGGGCA30750.069.081634
Produced by FastQC (version 0.11.5)