FastQCFastQC Report
Mon 27 Feb 2023
SRR3128770.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128770.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1927117
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119110.618073526412771No Hit
CCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTG38290.19869058287587107Illumina PCR Primer Index 2 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGC36770.19080315310383333Illumina PCR Primer Index 2 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTG32960.1710326876883967Illumina PCR Primer Index 2 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT45600.078.0105061
AGGGATG48000.077.4484565
ACGGGTA2750.076.907484
CGTAGGG5600.076.369432
GTACGGG5550.073.670312
TAGGGTA7000.073.184184
TAGAGGG35900.072.654872
GGATGGC35000.071.8394857
GAATAGG12850.071.771021
GGTAAGG11500.071.603851
GAGGGAT32850.071.392724
AGTAAGG13300.070.757831
AGAGGGC24850.070.734933
AAGAGGG71800.070.4294052
AAGGGAT41700.070.104044
AAGGGTA17900.069.842114
AGGGTAT13800.069.815565
CGAAGGG19000.069.752812
AAGGGCA31600.069.45744
TAAGGGA19500.069.4139253
Produced by FastQC (version 0.11.5)