FastQCFastQC Report
Mon 27 Feb 2023
SRR3128769.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128769.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1956950
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124200.6346610797414344No Hit
CTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGC33440.17087815222667926TruSeq Adapter, Index 15 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTG31470.1608114668233731No Hit
GCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTG26110.13342190653823552No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC20490.10470374817956514No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT59250.079.235981
ACGGGTA2150.074.569434
AGGGATG28300.073.395045
GGTAAGG11800.071.574571
CGTAGGG4700.071.232942
GTAGGGT6900.070.3895953
TAAGGGA17100.070.041913
ATAGGGC10900.069.649783
ATAGGGA16400.069.006153
AAGGGAT32950.068.978224
AGAGGGC25400.068.132253
TAGAGGG34300.067.7755662
GTAGGGA14250.067.504963
ATAGAGG12150.067.18271
AAGAGGG70550.067.172172
AGTACGG2400.066.842911
GAGGGAT26600.066.654054
AGTAGGG29300.066.627432
TAGGGAT11800.066.335564
TAGGGTC5700.066.1813354
Produced by FastQC (version 0.11.5)