FastQCFastQC Report
Mon 27 Feb 2023
SRR3128768.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128768.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2011816
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121740.605124921961054No Hit
CTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGC36530.1815772416562946TruSeq Adapter, Index 15 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTG34050.16925007058299565No Hit
GCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTG28460.14146422933310004No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT58400.080.419111
TAGACGG2500.073.411851
AGGGCAT15600.071.40095
TAAGGGA17650.070.0311053
TAGGGTA6050.069.914474
AGTAGGG33900.069.497042
ATAGAGG12800.069.4853741
AAGGGAT34200.069.397724
TATAGGG15700.069.189562
TAGAGGG33600.068.99792
AGTAAGG12150.068.554911
AGGGATG29950.068.104045
AGGGATA24150.067.723915
AGAGGGC28550.067.4928363
GTAGGGC9700.067.347733
GTAGGGA15250.067.184113
ATAGGGA19600.066.90033
ATAGGGT8550.066.511883
ACGGGTA2200.066.224544
TAGGGTC5750.066.205964
Produced by FastQC (version 0.11.5)