FastQCFastQC Report
Mon 27 Feb 2023
SRR3128767.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128767.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2122276
Sequences flagged as poor quality0
Sequence length100
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125050.5892259065267665No Hit
CTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGC39100.184236169093935TruSeq Adapter, Index 15 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTG35550.167508844278501No Hit
GCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTG29660.13975562085233023No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC21350.100599545016765No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT58150.077.564841
CGTAGGG6200.074.2800452
TATAGGG17950.072.781012
TAGGGTA6150.071.827464
GGTAAGG12450.071.39841
AGGGATG29700.071.36055
AAGGGAT35250.070.656884
ATAGGGA18700.070.61593
ATAGAGG13300.069.664351
AGTACGG2500.069.607771
AAGAGGG78300.068.719742
TAAGAGG14700.068.148811
GAGGGAT29250.068.120484
AGAGGGA51200.068.103863
TAAGGGA19200.067.797913
ATAGGGC12700.067.715043
TAGAGGG36000.067.4878852
GTAGGGA17000.067.173063
AGTAAGG13550.066.990621
AGGGCAT17450.066.787325
Produced by FastQC (version 0.11.5)