FastQCFastQC Report
Mon 27 Feb 2023
SRR3128766.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128766.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1893603
Sequences flagged as poor quality0
Sequence length100
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142800.7541179434126372No Hit
CCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTG25480.13455829970696076TruSeq Adapter, Index 13 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGC25240.13329087459198152TruSeq Adapter, Index 8 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTG22900.12093347972093411TruSeq Adapter, Index 13 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT54250.079.580561
ATAGGGC12600.074.10743
TAAGGGA17550.073.6275563
TAGGGCA12100.072.492744
TAGGGTA6200.072.2601554
ATAGGGA16700.071.727263
GAATAGG10500.070.99921
CGTAGGG5650.070.947552
TAGAGGG33250.070.632512
GTAGGGC7850.070.2883153
AGGGAAT20650.070.090355
AGGGATG31100.069.959745
AGTAGGG30350.068.835432
GGTAAGG14250.068.208421
AGATAGG12600.068.153241
ATAGAGG13600.067.6521151
AGGGTAC9600.067.5515755
AAGAGGG67300.066.359312
AGGGCAT16350.066.105515
ATAGCGG3800.065.8078841
Produced by FastQC (version 0.11.5)