FastQCFastQC Report
Mon 27 Feb 2023
SRR3128765.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128765.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1926914
Sequences flagged as poor quality0
Sequence length100
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139100.7218796479759865No Hit
CCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTG27360.14198869280102797TruSeq Adapter, Index 13 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGC26610.13809645889749103TruSeq Adapter, Index 8 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTG23210.12045166520145684TruSeq Adapter, Index 13 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGGT1150.081.735163
CGTTTTT51200.079.697771
CGTAGGG5150.077.607312
AGGGATG28550.074.735425
GAATAGG11850.072.682491
TAGGGCA9050.070.6263964
GTAGGGC8350.070.3558653
GTACGGG5450.069.884282
GGTAAGG12850.069.59011
AGTAGGG30800.068.699362
AAGAGGG65850.067.1931152
ATAGGGC11550.067.1395953
AGGGAAT17250.067.022835
AGTAAGG12500.067.020391
ACGGGTA2250.066.8411944
AAGGGAT32750.066.5860754
ATAGGGA16500.066.366483
AGATAGG12450.065.7774351
GAGGGAT20950.065.72954
GTAAGGG31550.065.2778242
Produced by FastQC (version 0.11.5)