FastQCFastQC Report
Mon 27 Feb 2023
SRR3128764.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128764.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2017115
Sequences flagged as poor quality0
Sequence length100
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142850.7081896669252868No Hit
CTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGC29690.14719041799798227TruSeq Adapter, Index 8 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTG29240.1449595090017178TruSeq Adapter, Index 13 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTG25620.127013085520657TruSeq Adapter, Index 13 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT55800.079.5205461
CGTAGGG5900.075.673812
AGGGATG30600.072.796325
GAATAGG12500.070.770091
TACGGGT1600.070.496133
AGTAAGG12400.070.202391
GTAGGGC8200.069.922993
TAGGGCA9850.069.6611564
ATAGGGA17450.069.486163
AGGGCAT17400.068.6020365
AGTAGGG31950.068.5471042
AGGGTAC7850.068.247655
ATAGGGC10000.068.1462553
GGTAAGG15250.067.882011
AAGAGGG72550.067.694432
ACGGGTA2500.067.6762854
AGATAGG11950.067.333331
TAAGGGA16650.066.614973
AAGGGCA30650.066.547744
GTAGGGA15550.066.491533
Produced by FastQC (version 0.11.5)