FastQCFastQC Report
Mon 27 Feb 2023
SRR3128763.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128763.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2400397
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT176690.7360865723461577No Hit
CTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTGC32880.13697734166473297Illumina Single End Adapter 1 (95% over 21bp)
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC32210.1341861367098859No Hit
CCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTG31720.13214480771305748No Hit
GCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTG27520.11464770202595655No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT71900.081.03131
TAGGGTA6800.077.669654
CGTAGGG6100.075.759742
ATAGGGA23500.074.447193
AGGGATG40200.072.711355
GAATAGG14950.071.946291
ATAGCGG4900.071.244351
AGGGAAT23950.071.0298545
TAGGGCA8900.069.940094
AGTAGGG34550.069.199372
ATAGAGG17400.069.136231
AGGGCAT20000.069.080475
ATAGGGC12650.068.964163
ACGGGTA3100.068.4530944
GTAGGGT8350.068.334683
TAGAGGG46350.067.657222
GTAGGGC9650.067.436353
TATAGGG18400.067.4031452
CGAAGGG22950.067.395892
TAGGGAT18800.067.22324
Produced by FastQC (version 0.11.5)