FastQCFastQC Report
Mon 27 Feb 2023
SRR3128762.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128762.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2438462
Sequences flagged as poor quality0
Sequence length100
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT171990.7053216330621515No Hit
CTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTGC34700.14230281218243301Illumina Single End Adapter 1 (95% over 21bp)
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC32720.13418293990228267No Hit
CCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTG31920.13090218342545423No Hit
GCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTG29660.12163404637841393No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT70100.083.228721
CGTAGGG7500.078.368582
TAGGGTA6600.077.616334
GAATAGG15550.075.039661
ATAGGGA24600.073.743333
ATAGCGG4800.073.51731
AGGGATG39750.072.712415
GTAGGGT8700.070.225473
GTACGGG4850.068.835082
AGTAGGG40450.068.119642
AGGGAAT24450.067.660375
GGAGCTA52600.067.636449
AGGGTAC9800.067.61825
TAAGGGA20500.066.942143
TAGAGGG44550.066.916752
GTAGGGA20350.066.742743
TAGGGCA9450.066.641324
TAAGAGG17450.066.329881
TAGGGAT19900.066.12654
CGAAGGG22700.066.078182
Produced by FastQC (version 0.11.5)