FastQCFastQC Report
Mon 27 Feb 2023
SRR3128761.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128761.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2566027
Sequences flagged as poor quality0
Sequence length100
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT178510.6956668811357012No Hit
CTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTGC35130.13690424925380754Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTG33750.13152628557688598No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC33110.1290321574948354No Hit
GCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTG30880.12034167995894042No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT67850.080.690471
ATAGCGG4650.076.874051
TAGGGTA6800.076.711544
CGTAGGG6350.076.227232
AGGGTAC11050.071.02335
GAATAGG16850.070.90111
AGGGATG41700.070.322655
TAGGGCA10850.070.166834
GGAGCTA65000.069.5517969
AGTACGG3050.069.3955841
ATAGGGA20600.069.122913
CGAAGGG26150.068.649642
AGATAGG14750.068.559171
AGGGAAT25100.068.3385545
TAGAGGG46800.068.182122
AGTAGGG43850.067.83922
ATAGGGC13750.067.330243
GTACGGG7100.066.8512652
AAGAGGG96150.066.7159962
GTAGGGC10850.066.7018053
Produced by FastQC (version 0.11.5)