FastQCFastQC Report
Mon 27 Feb 2023
SRR3128760.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128760.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2502041
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT274971.0989827904498766No Hit
CCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTG35680.14260357843856275No Hit
CTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGC35060.1401256014589689No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT112500.084.280491
AGGGATG42800.074.224075
TAGGGCA10900.072.6385964
AGTAGGG35200.072.031812
GTAGGGC9600.070.2023
AAGGGAT44150.068.638054
TAAGGGA21500.068.39133
ATAGGGC14100.068.186083
AAGAGGG86750.068.071652
AGTAAGG16150.068.022231
AGAGGGC37300.067.9763953
ATAGGGA22800.067.799063
GGTAAGG15250.067.39911
CGTAGGG5250.066.42892
ATAGAGG16200.066.357091
TAGAGGG44250.066.246322
GAGGGAT32850.066.137874
GGATAGG16850.065.47621
AAGGGCA35800.065.4271554
AGAGGGA54700.065.308023
Produced by FastQC (version 0.11.5)