FastQCFastQC Report
Mon 27 Feb 2023
SRR3128759.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128759.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2583467
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT274551.0627192063997721No Hit
CCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTG39090.1513083000479588No Hit
CTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGC38090.14743753258702355No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT108550.083.795481
AGGGATG43550.071.1149145
TAAGGGA20500.070.379783
TAGGGCA12100.069.5234154
GAATAGG15250.068.1931841
AGGGAAT23200.068.063575
GTACGGG6850.067.9637152
TAGGGTC8300.067.946424
ATAGGGA23850.067.587933
AAGAGGG91300.067.3704452
AAGGGAT46100.067.181234
TAGAGGG47600.067.179142
AGAGGGC36950.066.774143
ATAGGGC15850.066.713963
CGTAGGG6850.066.5907062
AGTAAGG17200.066.480821
GGTAAGG16800.066.103011
AGTAGGG40300.066.04562
TATAGGG19900.065.9300842
GTAGGGC10500.065.794783
Produced by FastQC (version 0.11.5)