FastQCFastQC Report
Mon 27 Feb 2023
SRR3128758.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128758.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2727947
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT281121.0305185547959692No Hit
CCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTG42020.15403525068485568No Hit
CTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGC40240.1475101972289051No Hit
GCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTG27540.10095504054880831No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT118950.083.484371
AGATAGG13700.070.0472261
GTAGGGC11950.069.999573
CGTAGGG7150.069.66832
AGGGATG41650.069.5038765
GGTAAGG15850.069.449321
GAATAGG13400.069.158061
ACGGGAT8250.068.9246754
TAGGGCA13300.068.9011154
ATAGGGC15700.068.8448643
AGTAGGG44400.068.690572
AGGGAAT25950.068.453835
TAGAGGG48450.066.634382
AAGGGAT41850.066.3644264
AAGAGGG101750.066.3216552
AAGGGTA21000.066.2392964
AGTAAGG16650.065.547351
TAAGGGA20800.065.2946853
ATAGAGG17950.064.99331
GATAGGG39350.064.8470842
Produced by FastQC (version 0.11.5)