FastQCFastQC Report
Mon 27 Feb 2023
SRR3128757.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128757.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1742409
Sequences flagged as poor quality0
Sequence length100
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108760.6241932864212708No Hit
CTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTGC30900.17734068178022497TruSeq Adapter, Index 15 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTG29040.16666580578957063No Hit
GCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTG23970.13756815994407742No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT46200.078.599641
AGTAGGG29450.074.763712
TAAGGGA12450.074.224273
ATAGGGA13100.074.140453
AGATAGG10500.074.108231
TAGGGTA3500.074.089034
ATAGGGC9300.073.0026863
ATAGAGG12250.070.450941
ACGGGTA2150.070.173124
GGTAAGG10050.069.918491
AGGGTAA6400.069.7557145
GTAGGGT5700.069.480613
TAATAGG5400.068.119691
AGGGTAC11800.068.10055
ACTAGGG8750.067.353662
AAGGGAC18100.067.204814
AGTAAGG12450.067.046441
AATAGGG27350.066.713372
GAGGGTA10250.066.6965564
TAGAGGG32950.066.679112
Produced by FastQC (version 0.11.5)