FastQCFastQC Report
Mon 27 Feb 2023
SRR3128756.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128756.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1804641
Sequences flagged as poor quality0
Sequence length100
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108030.5986232164735258No Hit
CTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTGC31940.17698811010056847TruSeq Adapter, Index 15 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTG30770.1705048261676422No Hit
GCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTG25630.14202270700931652No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGAGG12350.075.824561
TAGGGTA4600.075.6059344
CGTTTTT49350.074.85251
AGATAGG9900.074.625671
TAAGGGA14850.074.374393
AGTAAGG12900.074.0508651
ATAGGGC10500.072.9592363
AGTTAGG5250.071.705841
AGTAGGG33200.071.531952
GGAGCTA50550.071.496899
TAGGGCA8500.070.773894
TAATAGG6250.070.020751
ATAGGGA12600.069.751423
ATAAGGG26300.069.1993562
AGGGTAC11750.069.1974645
TAGAGGG32550.068.6261142
GATAGGG26050.068.599782
AGGGTAG8300.068.515575
ACGGGTA1600.067.560034
GTAGGGT6200.067.465273
Produced by FastQC (version 0.11.5)