FastQCFastQC Report
Mon 27 Feb 2023
SRR3128755.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128755.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1897959
Sequences flagged as poor quality0
Sequence length100
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109920.5791484431433978No Hit
CTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTGC32820.1729225973796062TruSeq Adapter, Index 15 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTG32360.17049894123108034No Hit
GCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTG27270.14368065906587024No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT50700.075.249891
ATAGGGC10500.075.2049943
GGTAAGG10700.075.180531
GGAGCTA65200.074.101419
GAATAGG11150.071.724421
AGTTAGG5650.071.604951
AGGGTAC10650.071.492065
AGATAGG10850.070.672561
TAATAGG6250.069.999661
ATAGAGG12550.069.720781
TATAGGG15800.069.612222
ATAGGGA14200.069.180653
TAGGGCA8800.068.900494
GCATAGG5550.068.6570361
TAAGGGA14550.068.485653
TAGAGGG35700.068.463932
CGTAGGG6350.068.0990142
AGTAGGG34650.068.096882
AAGGGAC18750.067.933394
ATAAGGG27250.067.787992
Produced by FastQC (version 0.11.5)