FastQCFastQC Report
Mon 27 Feb 2023
SRR3128754.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128754.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1937063
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127770.659606837774507No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC33680.17387147449515064No Hit
CTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTGC32070.16555992241863068No Hit
CCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTG30780.15890035584800288No Hit
GCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTG25280.13050685496548126No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT60100.081.3971561
ATAGGGC10750.078.5241553
TAGGGTC6100.078.081884
AGGGATG35700.075.030545
ACGGGTA3150.074.854544
AGGGTAC8550.073.649555
TAGAGGG37400.073.3854752
TAGGGCA11150.073.169484
CGTAGGG5350.072.28012
AAGGGTA13050.072.273354
GAATAGG11950.071.846971
GATAGGG27200.071.08432
ATAGGGA18800.069.734173
AGTAGGG31650.069.1357962
AAGAGGG73300.067.295562
TAAGGGA17100.067.290293
AGTAAGG13400.066.8888551
AGATAGG10250.066.73431
GTAAGGG30050.066.539582
ATAAGGG25500.066.206642
Produced by FastQC (version 0.11.5)