FastQCFastQC Report
Mon 27 Feb 2023
SRR3128753.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128753.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1976441
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124240.6286046484564932No Hit
CTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTGC34400.17405022462092215No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC33970.1718745968131606No Hit
CCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTG33160.16777632117528427No Hit
GCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTG26230.13271329627345313No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT58500.080.560821
ATAGGGC12500.080.085143
TAGGGCA10650.075.462094
AGGGATG36400.074.5000845
ACGGGTA2800.073.85454
ATAAGGG27800.073.222792
TAGAGGG37200.072.159972
TAAGAGG13850.071.654711
TAGGGTC4400.071.565614
AGGGTAC10050.071.549685
TAAGGGA18450.071.3253563
AGTAAGG12550.069.707781
GACCGAT1350.069.627148
ATAGAGG15400.068.718531
GAGGGTA12950.068.592154
AAGAGGG74150.068.218842
ATAGGGA18950.068.203363
AGAGGGC29900.068.061113
TAGGGTA6650.067.84724
GAATAGG12450.067.623211
Produced by FastQC (version 0.11.5)